Revista de Ingeniería

revinge | eISSN 2011-0049 | ISSN 0121-4993

A Model of Extreme Normovolemic Hemodilution in the Conscious Swine to Evaluate Resuscitation Fluids

No. 36 (2012-01-01)
  • Juan David Pérez Coronado
    (1) MD. MSc Ciencias Biomédicas, jd.perez31@uniandes.edu.co
  • Sergio David Galvis Forero
    (2) Médico Veterinario, Universidad de la Salle, sd.galvis26@uniandes.edu.co
  • Andrew Akbar Shalá Vásquez
    (3) MSc en Ciencias Biomédicas Universidad de los Andes. Bogotá, Colombia, a-shala@uniandes.edu.co
  • Jaime Ramón Cabrales Arévalo
    (4) MD, especialista en cardiología y hemodinamia. Fundación Cardioinfantil-Instituto de Cardiología. Bogotá, Colombia. jcabrales@cardioinfantil.org
  • Pedro Josué Cabrales Arévalo
    (5) PhD. MSc Ingeniería Biomédica. Instituto de Bioingeniería La Jolla. California, Universidad de California, San Diego. pcabrales@ucsd.edu
  • Juan Carlos Briceño Triana
    (6) PhD Ingeniería Biomédica, Ingeniero Mecánico. Profesor Titular, Universidad de los Andes. Bogotá, Colombia. jbriceno@uniandes.edu.co
https://doi.org/10.16924/revinge.36.3

Abstract

A model of ENH (Extreme Normovolemic Hemodilution) in the conscious swine was developed. A 4 level blood exchange was performed down to 6% hematocrit. In each level 40% of total blood volume was withdrawn and replaced with the same volume of hydroxyethyl starch. Below hemoglobin (Hb) 3g/dl and 2 hours after last exchange there was tissue hypoxia. 4 of 10 animals survived and only Hb after instrumentation had statistical difference with Hb <10.4 g/dl in non-surviving animals. During ENH there was tissue hypoxia, increase heart rate and cardiac output, divergent blood pressure, decrease oxygen delivery and systemic vascular resistance.

Keywords: Experimental model, swine, conscious, normovolemic hemodilution, normovelemic, perfluorocarbon, hemosubstitute