Revista de Ingeniería

revinge | eISSN 2011-0049 | ISSN 0121-4993

Lacease Alginate Encapsulation: Comparison between Assisted and Non-assisted Extrusion for Large-scale Production

No. 39 (2013-07-01)
  • Juan C. González
    (1) * Ingeniero Químico. jc.gonzalez141@uniandes.edu.co
  • Adriana P. Ascencio
    (2) * Ingeniera Electrónica y Eléctrica. ap.ascencio266@uniandes.edu.co
  • Camila A. González-Williamson
    (3) * Ingeniera Electrónica y Eléctrica. ca.gonzalez961@uniandes.edu.co
  • Johann F. Osma
    (4) * Ph.D en Ingeniería Química Ambiental y de Procesos. Profesor Asistente. jf.osma43@uniandes.edu.co
https://doi.org/10.16924/revinge.39.4

Abstract

The effect of assisted and non-assisted extrusion for large-scale production on size, shape and biochemical performance of laccase-alginate beads was studied. Results showed that the extruding technique affects bead size and size distribution but not the shape of the beads. Biochemical characterization showed a similar performance for both extruding methods. However, non-assisted beads presented higher enzymatic activity variability and lower stability in time. Results showed that using the extrusion method, bioprocesses are improved by highly homogeneous particulate material facilitating temperature or pH controlled steps. Thus, an assisted method presents several benefits for producing laccase particulate material in large quantities.

Keywords: Encapsulation, laccase, extrusion